A TECHNOLOGY FOR CULTIVATION OF HIGHLY ACTIVE RESISTANT MICROORGANISMS CAPABLE OF DEGRADING ENVIRONMENTAL ANTHROPOGENIC POLLUTANTS

The technology is intended to produce biomass of oil-oxidizing strains such as Acinetorbacter sp. HB-1, Mycobacterium flavescence EX-91 and Pseudomonas sp. strain5. The technology emplies the use of the association of cultures to utilize different oil pollutants.

• the composition of enriched mineral media providing high yield of final cultures is developed;
• temperature-time regime of cultivation is tried out;
• requirements to mass-exchange characteristics of the equipment are established;
• a regime for introduction of nutrient additives into cultures is studied, the process is tested;
• the process is scaled up for apparatus with capacity of l m³;
• cultures obtained according to these regimes are shown to retain their oil-oxidizing activity at the level similar to that of an initial strain; environmental preparations based on the are produced;
• the association of cultures produced by this technology is successfully trailed in biodegradation of recalcitrant oil products (oils).

• large-scale production of the preparation for remediation of oil polluted soil and water environment as well as for cleanup grease-stained water and hard surfaces;
• production of native cultures for on-site bioremediation;
• the use of science-methodological approaches to the development of processes to create strains degrading other types of pollutants.

• oil-extracting, machine engineering and other industries.

• laboratory instructions related to storage of working strains as well as to the production of oil-oxidizing cultures;
• technical requirements to cultures as well as drafts of regulations for pilot-industrial production of oil-oxidizing cultures;
• application for invention "the procedure to cultivate of highly active oil- oxidizing microorganisms".

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