Recombinant strains of Pseudomonas spp. were constructed for the development of paint-protection preparations against Coleoptera insects.

These strains are adaptable for manufacturing (they have a high growth rate, the components of nutrient media for their cultivation are accessible), show Coleoptera-specific insecticide activity, which is determined by Bacilus thuringiensis subsp. tenebrionis gene. In contrast to B.thuringiensis, crystalline protein synthesized by recombinant Pseudomonas, is localized inside the cell, thus being protected by the cell envelope from the influence of a number of damaging environmental factors. Negative effect of the endotoxin of the liability of the recombinant strains was decreased due to the system of induced cryIII-gene expression. The use of recombinant Pseudomonas strains as advanced enthomotoxin producers is thus determined by the properties of recipient strains and possibility to optimize the procedure of cultivation. Production of delta-endotoxin by recombinant Pseudomonas strains reaches 60-70% of the total amount of cell protein. However, the induction of cryIII-determinant expression is Pseudomonas may result in the inhibition of cell population growth and destabilization of the recombinants and toxin synthesis induction.

The preparation of a patent (Application N 95119335) for P.putida IPM-36 as a producer of incapsulated bioinsecticide against Colorado Beatles and for me technology of its construction is in progress now.

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